Free Peptide Reconstitution Calculator

Calculate exact syringe measurements for any peptide dose — instant results, no signup required

1

Syringe Size

2

Peptide Amount (mg)

3

Bacteriostatic Water (mL)

4

Desired Dose (mcg)

Your Result

Draw from your mixed vial

units

mL of reconstituted peptide

Concentration

mg/mL

Doses per vial

doses

Draw exceeds syringe capacity

You need mL but your syringe holds only mL. Use a larger syringe or add more bacteriostatic water to reduce concentration.

Enter valid values above to see your result.

How to Use This Calculator

Select the insulin syringe you will be using. The most common sizes are 0.3 mL (30 units), 0.5 mL (50 units), and 1.0 mL (100 units). If your dose is small, a 0.3 mL syringe provides better accuracy for reading the markings.

Enter the total amount of peptide in your vial in milligrams (mg). This is printed on the vial label. Common amounts are 5 mg, 10 mg, 15 mg, and 20 mg. You can also type a custom value if your vial size is not shown.

Enter the amount of bacteriostatic water (also known as BAC water, or reconstitution solution) you added or plan to add to the vial. Adding more water makes the solution more dilute, which means you draw more volume per dose but can measure more precisely. Also keep in mind that a standard peptide vial (98% of peptides are this size) can only hold a max of 3 mL of liquid, unless it is a large vial like NAD+ in which case it can hold up to 10 mL of liquid.

Enter the dose you want to inject in micrograms (mcg). This depends on the specific peptide and your protocol. For example, BPC-157 is commonly dosed at 250-500 mcg, while Tirzepatide starts at 2.5 mg (2,500 mcg). Refer to your protocol for the correct dose. If your dose is in milligrams (mg), you will need to convert it into mcg. 1 mg = 1,000 mcg.

The result panel shows the exact number of units to draw on your syringe, the equivalent volume in mL, the concentration of your mixed solution, and how many total doses you can get from the vial. The syringe graphic updates in real time to show where you should draw to. If you see a red warning, your dose exceeds the syringe capacity and you need to adjust your inputs.

Peptide Reconstitution Guide

What Is Peptide Reconstitution?

Peptide reconstitution is the process of mixing a lyophilized (freeze-dried) peptide powder with a sterile solvent, typically bacteriostatic water, to create an injectable solution. Most research peptides are sold as a dry powder in sealed vials and must be reconstituted before use. The process involves slowly adding the water to the vial, allowing the peptide to dissolve without shaking or agitating, which can damage the delicate molecular structure.

Why Accurate Dosing Matters

Precision in peptide dosing directly impacts both safety and effectiveness. Under-dosing may produce no results, while over-dosing can lead to unwanted side effects or waste expensive material. The concentration of your reconstituted solution depends on how much water you add: more water means a more dilute solution, making it easier to measure small doses accurately with standard insulin syringes. Using a reconstitution calculator eliminates math errors and ensures consistent dosing every time.

Common Peptides and Typical Doses

Peptide Typical Dose Common Vial Size
BPC-157 250 - 500 mcg 5 mg / 10 mg
TB-500 500 - 1,000 mcg 5 mg / 10 mg
Tirzepatide 2,500 - 15,000 mcg 5 mg / 10 mg / 20 mg / 30 mg / 40 mg / 50 mg / 60 mg
Retatrutide 1,000 - 10,000 mcg 5 mg / 10 mg / 20 mg / 30 mg / 40 mg / 50 mg / 60 mg
CJC-1295 / Ipamorelin 200 - 400 mcg 10 mg
KLOW Blend 3,000 mcg 80 mg

Note: Doses listed are extracted from popular protocols. Always follow your specific protocol and consult a healthcare professional.

Storage Tips

  • Unreconstituted peptides (in powder form) can be stored at room temperature for short periods but last longest when kept frozen (-20 °C).
  • Reconstituted peptides (peptides in liquid form) must be refrigerated (2-8 °C) and used within 4-6 weeks for most peptides. Never freeze a reconstituted vial.
  • Keep away from light. Peptides degrade with UV exposure.
  • Use bacteriostatic water or reconstitution solution (not sterile water or saline) for reconstitution, as the benzyl alcohol preservative helps prevent bacterial growth during multi-dose use.

Frequently Asked Questions

Enter four values: your syringe size, the amount of peptide in the vial (in mg), the volume of bacteriostatic water you added (in mL), and the dose you want (in mcg). The calculator instantly shows how many units to draw on your insulin syringe, along with the concentration and total doses per vial. No math required.

There is no single correct amount. Common practice is to add 1-3 mL of bac water depending on your desired level of dilution as well as the size (amount) of your individual injection. Adding more water makes each dose a larger volume, which is easier to measure accurately but means drawing and injecting more liquid. Adding less water creates a more concentrated solution which can prevent certain peptides from fully dissolving and even if fully dissolved may cause injection site irritation for some users due to the concentration of the peptide. Use this calculator to experiment with different water volumes and see how the syringe draw changes.

Insulin syringes are marked in "units" based on the U-100 standard, where 100 units equals 1 mL. So 10 units = 0.1 mL, 50 units = 0.5 mL, and so on. When using peptides (not insulin), the "units" are simply a convenient way to measure volume. This calculator converts your desired peptide dose directly into syringe units so you can draw accurately.

Yes. This calculator works with any peptide that is reconstituted with bacteriostatic water and injected with an insulin syringe. It supports BPC-157, TB-500, Semaglutide, Tirzepatide, CJC-1295, Ipamorelin, GHK-Cu, PT-141, DSIP, Epithalon, and any other lyophilized peptide. The math is the same regardless of the peptide type.

Absolutely. This peptide reconstitution calculator is 100% free with no signup or account required. Use it as many times as you need. If you want additional features like custom protocol creation, saved protocols, dose tracking, shot reminders, and access to our AI peptide expert, check out our My Peptide Pal app (free trial).

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